Evaluation of antimicrobial activity in contaminated root canals and cytotoxicity of ozonized products: an ex-vivo study
Avaliação da atividade antimicrobiana em canais radiculares contaminados e citotoxicidade de produtos ozonizados: um estudo ex-vivo
DOI:
https://doi.org/10.53660/CLM-3335-24H42Palavras-chave:
Antimicrobials, Endodontics, Ozone, Ozonized oilsResumo
Pulp and periapical diseases originate from microbial colonization of root canals. The development of new technologies and instrumentation techniques has provided more effective shaping of the root canal system. Even so, sodium hypochlorite and calcium hydroxide remain examples of auxiliary substances for endodontic treatment. Recently, ozone has emerged as promising in endodontic therapy. This study evaluated the antimicrobial activity of ozonized water and ozonized oils in root canals infected with Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans, in addition to the cytotoxicity of ozonized products. 224 human lower premolars were used, divided into 4 groups according to the microorganism tested, challenged with ozonized medications and compared to sodium hypochlorite and calcium hydroxide. The antimicrobial activities of ozonized oils were comparable to those established by sodium hypochlorite and calcium hydroxide. Ozonated sunflower oil and olive oil exhibited lower cytotoxicity and showed promise for understanding new metabolic routes and additional approaches in Endodontics.
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ABUHAIMED, T. S.; ABOU NEEL, E. A. Sodium Hypochlorite Irrigation and Its Effect on Bond Strength to Dentin. BioMed Research International, v. 2017, p. 1930360, 2017.
ALSHANTA, O. A. et al., Candida albicans biofilm heterogeneity and tolerance of clinical isolates: Implications for secondary endodontic infections. Antibiotics, v. 8, n. 4, p. 204, 2019.
ANZOLIN, A.; DA SILVEIRA-KAROSS, N.; BERTOL, C. Ozonated oil in wound healing: What has already been proven? Medical Gas Research, v. 10, n. 1, p. 54–59, 2020.
BANERJEE, A. et al., Insights into the ameliorative effect of oleic acid in rejuvenating phenylhydrazine induced oxidative stress mediated morpho-functionally dismantled erythrocytes. Toxicology Reports, v. 7, p. 1551–1563, 1 jan. 2020.
BERENJI, F. et al., Comparing the effect of ozonized olive oil with clotrimazole on three Candida species C. albiacans, C. glabrata, C. krusei. E3 J Microbiol Res, v. 2, n. 1, p. 9-13, 2014.
BLATTES, G. B. F. et al., Cell migration, viability and tissue reaction of calcium hypochlorite based-solutions irrigants: An in vitro and in vivo study. Archives of Oral Biology, v. 73, p. 34–39, 2017.
BOCH, T. et al., Effect of gaseous ozone on Enterococcus faecalis biofilm–an in vitro study. Clinical oral investigations, v. 20, p. 1733-1739, 2016.
BORGES, G. Á. et al., In vitro evaluation of wound healing and antimicrobial potential of ozone therapy. Journal of Cranio-Maxillofacial Surgery, v. 45, n. 3, p. 364-370, 2017.
CHÁVEZ-ANDRADE, G. M. et al., Antimicrobial and biofilm anti-adhesion activities of silver nanoparticles and farnesol against endodontic microorganisms for possible application in root canal treatment. Archives of oral biology, v. 107, n. July, p. 104481, 2019.
DEDHIA, J. et al., Efficacy of Andrographis paniculata compared to Azadirachta indica, Curcuma longa, and sodium hypochlorite when used as root canal irrigants against Candida albicans and Staphylococcus aureus: An in vitro antimicrobial study. Journal of Conservative Dentistry: JCD, v. 21, n. 6, p. 642, 2018.
DÍAZ, M. F. et al., Assessment of the physicochemical quality indicators and microbiological effects of Brazilian ozonized vegetable oils. Journal of the Brazilian Chemical Society, v. 32, n. 1, p. 216–224, 2021.
DUTTA, S. D.; MARIA, R. D. Homeopathic consideration for resistant endodontic bacteria Enterococcus faecalis: An in vitro comparative disc diffusion study. Journal of Conservative Dentistry: JCD, v. 23, n. 5, p. 528, 2020.
EL SAYED, M. et al., Antibacterial Activity of Sodium Hypochlorite Gel versus Different Types of Root Canal Medicaments Using Agar Diffusion Test: An In Vitro Comparative Study. International Journal of Dentistry, v. 2020, p. 1–11, 28 nov. 2020.
ELSHINAWY, M. I. et al., Synergistic Effect of Newly Introduced Root Canal Medicaments; Ozonated Olive Oil and Chitosan Nanoparticles, Against Persistent Endodontic Pathogens. Frontiers in Microbiology, v. 9, n. July, 2018.
FROUGH-REYHANI, M. et al., Antimicrobial efficacy of different concentration of sodium hypochlorite on the biofilm of Enterococcus faecalis at different stages of development. Journal of clinical and experimental dentistry, v. 8, n. 5, p. e480, 2016.
GLUSKIN, Alan H. et al. The double-edged sword of calcium hydroxide in endodontics: Precautions and preventive strategies for extrusion injuries into neurovascular anatomy. The Journal of the American Dental Association, v. 151, n. 5, p. 317-326, 2020.
GOZTAS, Z. et al., Antimicrobial effect of ozonated water, sodium hypochlorite and chlorhexidine gluconate in primary molar root canals. European journal of dentistry, v. 8, n. 04, p. 469-474, 2014.
GRANDI, G. et al., In vitro antimicrobial activity of ozonated oil in liposome eyedrop against multidrug-resistant bacteria. Open Medicine, v. 17, n. 1, p. 1057-1063, 2022.
HUBBEZOGLU, I. et al., Antibacterial Efficacy of Aqueous Ozone in Root Canals Infected by Enterococcus faecalis. Jundishapur Journal of Microbiology, v. 7, n. 7, p. 11411, 2014.
JIANG, Y. T. et al., Biological changes of Enterococcus faecalis in the viable but nonculturable state. Genetics and Molecular Research, v. 14, n. 4, p. 14790–14801, 2015.
KATEBI, K. et al., The comparison of calcium hydroxide, curcumin, and Aloe vera antibacterial effects on 6-week-old Enterococcus faecalis biofilm as an intracanal medicament: An in vitro study. Dental Research Journal, v. 19, n. 1, p. 14, 2022.
KIST, S. et al., Comparison of ozone gas and sodium hypochlorite/chlorhexidine two-visit disinfection protocols in treating apical periodontitis: a randomized controlled clinical trial. Clinical Oral Investigations, v. 21, n. 4, p. 995–1005, 2017.
KUMAR, D. et al., Association of specific microorganisms with endodontic signs and symptoms. A comparative study. Journal of Family Medicine and Primary Care, v. 9, n. 8, p. 3965, 2020.
LEI, L. et al., Exopolysaccharide dispelled by calcium hydroxide with volatile vehicles related to bactericidal effect for root canal medication. Journal of Applied Oral Science, v. 24, n. 5, p. 487–495, 2016.
LIAQAT, N. et al., Green synthesized silver nanoparticles: Optimization, characterization, antimicrobial activity, and cytotoxicity study by hemolysis assay. Frontiers in chemistry, v. 10, p. 952006, 2022.
MAEKAWA, L. E. et al., Effect of Zingiber officinale and propolis on microorganisms and endotoxins in root canals. Journal of Applied Oral Science, v. 21, n. 1, p. 25–31, 2013.
MARTINHO, F. C. et al., Clinical comparison of the effectiveness of 7- and 14-day intracanal medications in root canal disinfection and inflammatory cytokines. Clinical Oral Investigations, v. 22, n. 1, p. 523–530, 6 jun. 2017.
MATTOS, M. M. R. S. Uma nova abordagem para o tratamento de infecções endodônticas persistentes com o uso do extrato de aroeira bruto a 50% frente a uma cepa de enterococcus faecalis ATCC 29212. Instituto de Ciências da Saúde, 20 jul. 2017.
METO, A. et al., Antimicrobial and antibiofilm efficacy of a copper/calcium hydroxide-based endodontic paste against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Dental Materials Journal, v. 38, n. 4, p. 591-603, 2019.
MONZILLO, V. et al., Ozonized Gel Against Four Candida Species: A Pilot Study and Clinical Perspectives. Materials, v. 13, n. 7, p. 1731, 8 abr. 2020.
NIRWANA, I. et al., Antibacterial activity of fig leaf (Ficus carica Linn.) extract against Enterococcus faecalis and its cytotoxicity effects on fibroblast cells. Veterinary World, v. 11, n. 3, p. 342–347, 20 mar. 2018.
NOGALES, C. G. et al., Ozone therapy as an adjuvant for endondontic protocols: Microbiological – Ex vivo study and citotoxicity analyses. Journal of Applied Oral Science, v. 24, n. 6, p. 607–613, 2016.
NOGALES, C. G.; FERREIRA, M. B.; LAGE-MARQUES, J. L. Comparison of the antimicrobial activity of three different concentrations of aqueous ozone on Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis–in vitro study. Ozone Therapy Global Journal, v. 4, n. 1, p. 9-15, 2014.
NUNES, I. A. M. et al., Antimicrobial action of ozonated water and photodynamic therapy with sonic activation in root canals infected with Enterococcus faecalis. Journal of Clinical and Experimental Dentistry, v. 14, n. 6, p. 486–491, 2022.
OFFICIOSO, Arbace et al., Comparative analysis of the effects of olive oil hydroxytyrosol and its 5-S-lipoyl conjugate in protecting human erythrocytes from mercury toxicity. Oxidative Medicine and Cellular Longevity, v. 2018, 2018.
ÖZDEMIR, O. et al., The frequency of sodium hypochlorite extrusion during root canal treatment: an observational clinical study. Australian Dental Journal, p. 1–8, 2022.
PAROLIA, A. et al., Effect of propolis nanoparticles against enterococcus faecalis biofilm in the root canal. Molecules, v. 26, n. 3, p. 1–17, 2021.
PIETROCOLA, G. et al., Evaluation of the antibacterial activity of a new ozonized olive oil against oral and periodontal pathogens. Journal of clinical and experimental dentistry, v. 10, n. 11, p. e1103, 2018.
PINHEIRO, S. L. et al., Antimicrobial efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water as irrigants in mesiobuccal root canals with severe curvature of mandibular molars. European Journal of Dentistry, v. 12, n. 01, p. 094-099, 2018.
PRADA, I. et al., Update of the therapeutic planning of irrigation and intracanal medication in root canal treatment. A literature review. Journal of Clinical and Experimental Dentistry, v. 11, n. 2, p. e185–e193, 2019.
RAOOF, M. et al., Antimicrobial activity of methanolic extracts of Myrtus communis L. and Eucalyptus galbie and their combination with calcium hydroxide powder against Enterococcus faecalis. Journal of Dentistry, v. 20, n. 3, p. 195, 2019.
RATH, P. P. et al., The effect of root canal irrigants on dentin: a focused review. Restorative Dentistry & Endodontics, v. 45, n. 3, 2020.
RÔÇAS, I. N.; SIQUEIRA, J. F. Frequency and levels of candidate endodontic pathogens in acute apical abscesses as compared to asymptomatic apical periodontitis. PLoS ONE, v. 13, n. 1, p. 1–11, 2018.
RUKSAKIET, K. et al., Antimicrobial efficacy of chlorhexidine and sodium hypochlorite in root canal disinfection: a systematic review and meta-analysis of randomized controlled trials. Journal of endodontics, v. 46, n. 8, p. 1032-1041. e7, 2020.
SANTA-ROSA, C. C. et al., Estudo piloto da análise comparativa da atividade antimicrobiana da PDT e agentes irrigantes em canais radiculares inoculados com Enterococcus faecalis “in vitro”. Arquivos em Odontologia, v. 55, 2019.
SANTOS-JUNIOR, A. O. et al., Success or failure of endodontic treatments: A retrospective study. Journal of conservative dentistry: JCD, v. 22, n. 2, p. 129, 2019.
SARDA, R. A. et al., Antimicrobial efficacy of photodynamic therapy, diode laser, and sodium hypochlorite and their combinations on endodontic pathogens. Photodiagnosis and Photodynamic Therapy, v. 28, p. 265–272, dez. 2019.
SAVITRI, D. et al., Efficacy of Ozonated Water, 2% Chlorhexidine and 5.25% Sodium Hypochlorite on Five Microorganisms of Endodontic Infection: In Vitro: Study. Advances in Human Biology, v. 8, n. 1, p. 19-23, 2018.
SILVA, V. et al., High efficacy of ozonated oils on the removal of biofilms produced by methicillin-resistant staphylococcus aureus (MRSA) from infected diabetic foot ulcers. Molecules, v. 25, n. 16, 1 ago. 2020.
SONG, M. et al., The antibacterial effect of topical ozone on the treatment of MRSA skin infection. Molecular medicine reports, v. 17, n. 2, p. 2449-2455, 2018.
SOUZA, I. M. D. Produção, purificação e avaliação da citotoxicidade e atividade hemolítica do benzoato de benzila obtido por acilação mediada por Lipozyme® TL IM. 2022. 94p. Dissertação (Mestrado em Engenharia de Alimentos) – Universidade Federal de Santa Catarina.
TAHIR, A. et al., Influence of Sapindus mukorossi extract in comparison to 17% EDTA as final root canal irrigant on the sealer penetration and microleakage of dentinal tubules. European Review for Medical & Pharmacological Sciences, v. 27, n. 7, 2023.
TIBÚRCIO‐MACHADO, C. S. et al., The global prevalence of apical periodontitis: a systematic review and meta‐analysis. International Endodontic Journal, v. 54, n. 5, p. 712–735, 22 jan. 2021.
TIWARI, S. et al., Dental applications of ozone therapy: A review of literature. The Saudi Journal for Dental Research, v. 8, n. 1–2, p. 105–111, 1 jan. 2017.
VASAVADA, K.; KAPOOR, S. Evaluation of ozonized calcium hydroxide as an effective intracanal medicament during root canal procedures: An in vitro observational study. Medical Gas Research, v. 10, n. 3, p. 122–124, 1 set. 2020.
VERMA, D.; GARG, P. K.; DUBEY, A. K. Insights into the human oral microbiome. Archives of Microbiology, v. 200, n. 4, p. 525–540, 2018.
YADAV, P. et al., Evaluation of antimicrobial and antifungal efficacy of chitosan as endodontic irrigant against Enterococcus Faecalis and Candida albicans biofilm formed on tooth substrate. Journal of Clinical and Experimental Dentistry, v. 9, n. 3, p. e361–e367, 2017.
YANLING, C. et al., Efficacy of relacin combined with sodium hypochlorite against Enterococcus faecalis biofilms. Journal of applied oral science : revista FOB, v. 26, p. e20160608, 2018.
YASINI, Z. et al., Ex vivo efficacy of sonodynamic antimicrobial chemotherapy for inhibition of Enterococcus faecalis and Candida albicans biofilm. Photodiagnosis and Photodynamic Therapy, v. 40, p. 103113, 2022.
ZAKRZEWSKI, W. et al., The influence of ozonated olive oil-loaded and copper-doped nanohydroxyapatites on planktonic forms of microorganisms. Nanomaterials, v. 10, n. 10, p. 1997, 2020.
ZANCAN, R. F. et al., Antimicrobial activity of intracanal medications against both Enterococcus faecalis and Candida albicans biofilm. Microscopy research and technique, v. 82, n. 5, p. 494-500, 2019.
